BMD-08-peptide-01 BMD-10-abmuno BMD-07-01

Peptide合成

Peptide合成服務

胜肽合成服務

化學合成之胜肽可用來製備抗體或作為蛋白質定量的標準品,應用在蛋白質功能與結構之基礎研究、胜肽藥物研發與臨床治療的應用研究等。本公司提供多種合成量、純度及合成服務項目。

純度等級:Crude、Desalted、 >75%、>85%、>90%、>95%、>98%。
質量等級:5mg、10mg、15mg、20mg、25mg、30mg、40mg~1kg 以上,有多種等級。
合成時間:3~4週,依序列長短或修飾難易度

Peptide純度>75%以上, 皆有合成後分析報告。

Peptide合成服務內容:

  • 客製化 Peptide合成
  • Peptide 修飾服務
  • Peptide 胜肽應用

純度:

  • > Crude(50%)
  • > Desalted(60%)
  • >70%
  • >75%
  • >80%
  • >85%
  • >90%
  • >95%
  • >98%


合成項目:

簡單的、複雜的、線性的、環狀的、長序列多肽,可至140個胺基酸

修飾多胜肽 (Peptide Modification):

  1. N、C端修飾: N,C-Terminal Modification
  2. 特殊胺基酸: Special Amino Acid
  3. 螢光修飾標記: Fluorescence / Dye labeling (FITC等)
  4. 環狀胜肽: Cyclic Peptide
  5. 磷酸化多胜肽 (Quenched)
  6. 蛋白質結合 Protein Conjugation

1. N, C端修飾 (N,C-terminal Modification)

  • Acetylation (N-Terminal)
  • HYNIC (N-Terminal)
  • DTPA (N-Terminal)
  • Formylation (N-Terminal)
  • Fatty acid (N-Terminal)
  • Myristic acid (N-Terminal)
  • Palmytolyl (N-Terminal)
  • Benzyloxycarbonylation(CBZ)
  • Amidation (C-Terminal)
  • Succinylation (Suc, N-Terminal)

2. 特殊胺基酸合成(Special Amino acid) 

  • D-Arg
  • D-Cys
  • D-Asp
  • D-Asn
  • D-Glu
  • D-Gln
  • D-Ser
  • D-His
  • D-Thr
  • D-Trp
  • D-Leu
  • D-Ile
  • D-Met
  • D-Pro
  • D-Val
  • D-Phe
  • pGlu
  • Hyp
  • D-Lys
  • D-Tyr
  • D-Orn
  • Orn
  • Abu
  • Aib
  • (D)1-Nal
  • (D)2-Pal
  • (D)4-Cl-Phe
  • Nva
  • Nle
  • Hse
  • Hcy
  • Pen
  • Mpa
  • N-Methyl amino acid (Ala, Phe, Leu, Ile, Val, Gly, Met)
  • Other amino acid
  • Dinitrobenzoylation (Lys)
  • Lys(Me2)
  • Tyr(SO3H2)
  • Ser(octanoic acid)
  • Phosphorylation(Tyr, Ser, Thr, Single site)

3. 螢光修飾 Label (Fluorescence / Dye labeling)

  • Biotin (Lys in sequence)
  • Biotin (N-Terminal, Y/N Ahx)
  • Biotin (without Lys in sequence)
  • FITC/5-FAM (N-Terminal, Y/N Ahx)
  • Dansyl (N-Terminal, Y/N Ahx)
  • MCA (N-Terminal)
  • p-Nitroanilide(pNA, C-Terminal)
  • AMC (C-Terminal)

 4. 環狀胜肽 Cyclic Peptide

  • Disulfide bridg 1nd
  • Disulfide bridge 2nd
  • Disulfide bridge 3rd
  • Amide cyclic (Side chain, end)

5. 磷酸化多胜肽 Quenched Fluorescent Peptide

  • Abz/ Tyr (3-NO2)
  • EDANS/DABCYL 

6. Multiple Antigenic Peptide System

  • Asymmetric 4 branches
  • Asymmetric 8 branches

7. 蛋白質結合 Protein Conjugation

  • KLH
  • BSA

純度選擇

不同的實驗內容有不同的建議純度,如無法判斷請連絡本公司專案人員。 

Peptide 胜肽合成純度應用 >75%

  • Peptide Array and Screening Studies
  • Polyclonal Antibodies
  • Immunogenic Identification
  • Mutation Screening
  • Sequence Optimization
  • ELISA Tests 

Peptide 胜肽合成純度應用 >85% 

  • Phosphorylation Studies
  • Semi-quantitative Enzyme-Substrate Studies
  • Non-quantitative Peptide Blocking Studies for Western blotting and Immunocytochemistry
  • In-vitro Bioassays
  • Cell Attachment Studies
  • Epitope Mapping
  • Coupling to Chromatography Resins for Affinity Purifications
  • Antibody- Antigen Interaction Studies
  • Antigenic Peptides in Immune Lymphocyte Assays

Peptide 胜肽合成純度應用 >95% 

  • Quantitative Blocking and Competitive Inhibition Assays 
  • Quantitative Receptor-Ligand Interaction Studies
  • Quantitative Phosphorylation Studies & Quantitative Proteolysis Studies
  • In-vivo Studies & In-vitro Bioassays
  • Generating Standard Curves
  • Reference Standards & Diagnostic Kits
  • NMR spectroscopy Studies &Crystallography 

Peptide purity >98%

  • Clinical Trials & Drug Studies
  • SAR Studies

 

 胺基酸對照表

 

胺基酸

縮寫

Alanine

Ala

A

Arginine

Arg

R

Asparagine

Asn

N

Aspartic Acid

Asp

D

Cysteine

Cys

C

Glutamine

Gln

Q

Glutamic Acid

Glu

E

Glycine

Gly

G

Histidine

His

H

Isoleucine

Ile

I

Leucine

Leu

L

Lysine

Lys

K

Methionine

Met

M

Phenylalanine

Phe

F

Proline

Pro

P

Serine

Ser

S

Threonine

Thr

T

Tryptophan

Trp

W

Tyrosine

Tyr

Y

Valine

Val

V